Protemist DT II is a desktop robot for cell-free, hand-free, and maintenance-free protein production. With a protocol optimized for the wheat germ cell-free protein expression system, it performs transcription, translation, and batch affinity purification by itself in 35 hours.

It can run on either a 6-well format or a 24-well format to express up to 6 or 24 genes of interest. The robot produces up to 2 mg of proteins (crude) out of 1 ml of proprietary wheat germ extract, which translates to 2.5 to 3.0 mg of crude proteins per run. The batch affinity purification can be optimized by varying the temperature between 4 and 37C.
Cell Free Expression Consumables

 Instrument Capabilities

• Wheat germ cell-free protein synthesis
• Fully automated transcription and translation (bi-layer method) in less than 30 hours*
• Batch affinity purification for 6-well format
• 6-well or 24-well format by simple cartridge change
• Approx. 0.5mg/well (6-well format) or 0.1mg/well (24-well format) of crude protein production**
• Wide temperature control range for purification (4°C to 37°C)
• Control, record, and data retrieval by PC
• Disposable titer plates and tubes

*translation at 15°C
**GFP

Translation (6-ml format) with WEPRO 1240G at 15°C for 20hrs,
purification with glutathione Sepharose followed by elution with 1 ml reduction-type glutathione

M: Size marker
C: Crude sample (3µl)
E: Eluate (2µl)

Yield/well after purification