About the Author - Peter Nollert

Peter Nollert

I'm Peter Nollert and I write this blog to point researchers to topics that are relevant to protein crystallization. My mission is to help spread knowledge that is 'out there on the web' and help you succeed with your protein structure research.  I oversee the membrane protein research and technology development activities at Emerald BioStructures. Check out The GPCR blog, or my publications. 

Blog Archive

Protein Crystallization Hits

TitleReadFirstReadLastReadDaysAverage/Day
Protein Crystallization Conference ICCBM 13 live on the web74853/24/20125/23/2013425.2217.60
5 Rules of Crystal Cryopreservation for X-ray Diffraction38843/24/20125/23/2013426.179.11
Full transparency for all protein crystallization formulations from Emerald Bio: of course!26795/14/20125/23/2013375.027.14
Membrane Protein Crystallization with Additives in LCP25864/21/20125/23/2013397.16.51
Protein Crystallization phase diagrams25365/4/20125/23/2013385.116.59
EXTRACT! your membrane protein with best possible detergent 222312/29/20125/23/2013146.0615.22
Waiting the protein to concentrate: setting up your crystallization experiments for success20216/22/20125/23/2013336.126.01
Widening the protein production pipeline up-stream: Lysis scouting with the Protein Maker177210/17/20125/23/2013218.198.12
Rational Protein Crystallization Optimization Schema16573/30/20125/23/2013420.043.94
Recalcitrant Protein Crystallization by in-situ Proteolysis15083/31/20125/23/2013419.043.60
Best Practices for storing protein samples14355/16/20125/23/2013372.183.86
Protein Crystallization "Tips and Tricks" now in Acta Cryst F13085/16/20125/22/2013372.143.51
Hanging or sitting drops? Have it both ways12873/22/20125/23/2013428.183.01
New Detergent Class for Membrane Proteins12533/21/20125/23/2013429.12.92
Co-crystallization and crystal soaking for in-house SAD phasing: no magic necessary12293/22/20125/23/2013428.182.87
Seeding with protein 'crap': Microseed Matrix Screening11605/23/20125/23/2013366.023.17
Covering protein crystallization space Part II: which Buffers and Salts do you really need?11484/18/20125/23/2013400.172.87
Covering protein crystallization space: which PEGs do you really need?11174/18/20125/23/2013400.222.79
Simpler LCP-based Crystallization10653/26/20125/23/2013424.122.51
Beautiful protein crystal images9865/5/20125/23/2013384.092.57
Bicelle membrane protein crystallization method9803/31/20125/23/2013419.012.34
8 Practical Tips for Membrane Protein Crystallization9013/29/20125/23/2013420.162.14
Record 0.48 Angstrom X-ray crystallographic resolution for a protein8856/18/20125/23/2013339.212.61
Temperature scouting to increase protein crystallization success8725/1/20125/22/2013387.112.25
Beware of biochemists determining protein crystal structures?8658/14/20125/23/2013283.063.06
Protein Sample Preparation: Shoot for > 20 ODml85110/31/20125/23/2013204.134.17
Applying the 80/20 rule to membrane protein crystallization pre-screening8444/28/20125/22/2013390.132.16
How many stock solutions do you need to run an agile protein crystallization lab?8434/2/20125/22/2013416.142.03
Protein Crystallization by dehydration8383/23/20125/23/2013426.151.97
Protein crystal optimization with detergents8344/28/20125/23/2013391.022.13
Who needs crystallographers anyways?8184/28/20125/22/2013389.162.10
The Google for small molecule compounds7823/23/20125/23/2013426.221.83
Optimizing Membrane Protein Crystals with Lipids77410/8/20125/23/2013227.173.41
Disruptive Proteomics: the NIH wants your ideas7733/19/20125/23/2013430.051.80
5 min to Protein Crystallization Condition7555/1/20125/23/2013387.211.95
Protein Crystallization: The Art Delusion7475/2/20125/23/2013387.161.93
Our PlugMaker wins the New Product Award at LabAutomation!7284/19/20125/22/2013398.21.83
Plate material a source for ligand-complex crystallization failure?7234/30/20125/22/2013388.061.86
Shortening th path from protein sample to crystals7056/26/20125/23/2013331.222.13
Filtering out suitable membrane protein expression constructs7015/16/20125/23/2013372.221.88
Best Protein Crystallization Paper 20096674/26/20125/22/2013392.171.70
Growing monster-sized protein crystals6654/28/20125/22/2013389.111.71
X-FEL + in-vivo crystallization + crystal jets = game-changer in the make6655/20/20125/23/2013369.151.80
11 things I wish I had known about protein crystallization optimization when I set up my first optimization trays6627/13/20125/23/2013315.112.10
GPCR crystallography and what's next?6535/18/20125/23/2013371.041.76
Learning State-of-the-Art Cryocrystallography the Powerpoint way6433/28/20125/22/2013421.031.53
How-to: Protein Crystallization in an ideal world6425/2/20125/23/2013387.141.66
My first Iodide Soak (part I)6245/30/20125/22/20133581.74
Stability not required to grow protein crystals and: Ala Gly & Phe are your friends6215/16/20125/22/2013372.091.67
Best of 25 hottest articles from STRUCTURE6054/30/20125/23/2013389.021.56
Current Protein Crystallization Patent Applications5995/25/20125/22/2013363.121.65
Best of 2010 in Protein Crystallography5835/19/20125/23/2013370.211.57
Online 2D Optimization Matrix Pipetting Plan Calculator5823/26/20125/23/2013423.131.38
Risky business or, how much protein sample do you need?5778/20/20125/23/2013277.112.08
Choosing better buffers for crystallization56310/25/20125/23/2013211.212.67
Accidental protein crystallization (and structure!)5583/31/20125/23/2013418.11.33
Protein Crystallization Web Tools List5444/28/20125/23/2013390.091.39
Increasing the odds of protein crystallization success by protein engineering5414/28/20125/22/2013389.111.39
Protein Crystallization Drops: Stirred or shaken?5364/30/20125/23/20133891.38
Nobel prize for GPCRs!53610/10/20125/23/2013225.232.38
Avoiding the protein concentration step prior to setting up crystallization experiments5249/30/20125/23/2013235.12.23
Funniest Protein Crystallization paper ever5168/26/20125/23/2013271.081.90
Protein Crystallization in 20145116/5/20125/23/2013353.091.45
Soaks and co-crystallization not working? Try cross-linking50810/2/20125/23/2013233.222.18
Feeding Your Robot: Taking Chaos Out of Your Wet Lab 5044/26/20125/23/2013393.041.28
"Just Faking it" not working well for crystallographers5003/29/20125/22/2013420.141.19
Dirty protein - is purity overrated?5009/17/20125/23/2013249.072.01
Tips for Membrane Protein Crystallization50011/15/20125/23/20131902.63
Enzyme crystallization head start with BRENDA4924/28/20125/23/2013390.121.26
Filtration: simple sample pre-treatment for protein crystallization experiments4899/14/20125/23/2013251.191.95
Better late than never: the complete alpha helical membrane protein crystallization conditions database now online4825/9/20125/23/2013380.131.27
Iodide in-house phasing: Four new structures within a month!4826/27/20125/23/2013331.181.46
Slam dunk protein crystallizations4764/28/20125/22/2013390.081.22
Protein Crystallization with the MPCS rocks4646/5/20125/23/2013353.031.31
Ionic Liquids for Protein Crystallization4589/17/20125/22/2013248.11.85
Protein Crystallization Wiki4554/28/20125/23/2013390.161.17
Crystallization Game Changer: Try a Different Plate4505/16/20125/22/2013372.11.21
Better Protein Crystals with Ultrasound?4475/14/20125/22/2013374.031.20
2010 Protein Crystallization Nobel - cast your vote here!4414/28/20125/23/2013390.221.13
Protein Crystallization, biologics style4336/3/20125/22/2013354.031.22
Keeping up with the protein crystallography news flood: webinars and podcasts42111/1/20125/22/2013202.232.08
More plugs: simpler membrane protein crystallization with PLI41010/21/20125/22/20132141.92
Thank you!4035/20/20125/22/2013368.141.09
7 key elements to a successful protein crystallization day4004/28/20125/23/2013390.181.03
Plugs are the new drops3979/28/20125/22/2013237.121.67
Protein Crystallizers' Hall of Fame3819/13/20125/23/2013252.121.51
Protein Crystals: Of Pack-Men and Pigs3786/24/20125/23/2013334.081.13
Better Crystallization Cocktails for Beta Barrel Membrane Proteins37811/8/20125/23/2013196.21.93
Protein Crystallization Chaperones35910/25/20125/23/2013211.071.70
Need Crystals? Try Seaweed.3579/7/20125/22/2013258.011.38
Will my protein crystallize?34611/18/20125/22/2013185.171.87
Structures on a phone?3418/2/20125/23/2013295.151.16
Seeking Protein Crystallization Guidance3269/27/20125/23/2013238.071.37
The biggest SECRET ever for success in protein crystallization3139/6/20125/22/20132591.21
Six Simple Follow-on Experiments to Increase Your Crystallization Hit Rate On the Cheap3099/2/20125/22/20132631.17
Protein crystallization trial prepared in less than 10 min3049/2/20125/23/2013263.091.16
8 Reasons for Spring-Cleaning Your Protein Crystallization Lab2843/7/20135/23/201378.043.64
All drops clear? Back to Old School protein crystallization2839/7/20125/22/2013257.191.10
Newsflash: Nobel prize for the structure of the ribosome28210/6/20125/23/2013230.071.23
The protein-crowded cytoplasm: a Work of Art2823/27/20135/23/201358.14.85
Faculty of 1000 recommended "protein crystallization" + "tech advance" articles2599/24/20125/23/2013242.091.07
The new Emerald Bio25012/30/20125/22/2013143.141.75
Rescuing protein crystal hits with UV light2321/15/20135/23/2013129.041.80
Protein structures without growing bugs22711/19/20125/23/2013186.031.22
Cross-crystallization for optimization with metal ions21211/9/20125/22/2013195.081.09
Fragment Libraries: Buyer Beware2084/5/20135/23/201349.094.24
Warren DeLano19710/11/20125/23/2013225.050.88
Deficiency in Crystallographic Analysis: Brian Matthews sets the record straight18511/5/20125/23/2013200.030.92
Facing Protein Crystallizers' Remorse17711/19/20125/23/2013186.150.95
Great Expectations for PSI: Biology1731/31/20135/22/2013112.011.54
PDBsum rocks for crystallization protocol16311/19/20125/22/2013185.160.88
Two crystallization hits - what now?14412/10/20125/23/2013164.210.88
4 good reasons to grow protein crystals other than for X-ray diffraction1313/13/20135/23/201371.141.84
7 Ways to Maximize the Value of your Protein Crystallization Paper1222/21/20135/23/201391.231.34
Crystal Hits feedback for the timid10712/10/20125/22/2013163.040.66
Highlights from SLAS2012983/1/20135/22/201382.231.19
Best protein crystallization blog posts in 2010 so far773/6/20135/23/201379.160.97
The American Protein Recovery and Refolding Act623/27/20135/23/201357.21.08
Protein Crystallization for Beginners: my slide deck573/25/20135/22/201358.170.98
10 Ways to Spoil your Protein Sample Prior to Setting up a Crystallization Trial543/26/20135/22/201357.210.94
How to keep crystal images and crystallization conditions together505/2/20135/23/201322.092.26
Faster, cheaper detergent screening for MPs494/19/20135/23/201334.21.43
11 most popular 'Crystallization Hits' in 2010464/6/20135/23/201348.010.96
Higher throughput protein purification for crystallization success405/2/20135/23/201322.121.81
Lights out during crystallization?324/28/20135/22/201325.041.28
High Schmuput Crystallization?195/11/20135/23/201312.231.55
How to keep your protein crystallization papers organized65/21/20135/22/20131.234.88
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