by Peter Nollert
September 14, 2010 15:30
In case you have not heard about Emerald's newest protein crystallization tool, check out the MPCS - the Microcapillary Protein Crystallization System. This is an award winning, simple-to-use crystallization system that enables protein crystal growth with very little protein sample volume requirements (2.5 uL and you're in business). We have recently completed a side-by-side protein crystallization study and report our results in this paper:
Cory J. Gerdts, Glenn L. Stahl, Alberto Napuli, Bart Staker, Jan Abendroth, Thomas E. Edwards, Peter Myler, Wesley Van Voorhis, Peter Nollert and Lance J. StewartNanovolume optimization of protein crystal growth using the microcapillary protein crystallization systemJournal of Applied Crystallography (2010). 43
This is what the MPCS delivered in terms of successful crystallizations:
- 28 out of 29 (93%) proteins crystallized as compared to traditional vapor diffusion experiments
- 90 out of 120 (75%) protein/precipitant combinations lead to initial crystal hits from vapor diffusion experiments
- Many of the resulting crystals produced high-quality X-ray diffraction data, leading to six novel protein structures that were derived from crystals harvested from MPCS CrystalCards.
Protein crystallization study using the Plugmaker instrument and Crystal Cards.
The MPCS rocks,
Peter