October 20, 2010 04:03
A while ago Luc posted this useful comment to as a response to the blog post "Stability not required to grow protein crystals and: Ala, Gly & Phe are your friends":
There is already a very useful webserver to help you design mutants with better chances of crystallization, The Surface Entropy reduction Prediction Server.
Reference: Lukasz Goldschmidt, David Cooper, Zygmunt Derewenda, David Eisenberg. (2007).
Toward rational protein crystallization: A Web server for the design of crystallizable protein variants
Protein Science. 16:1569-1576 (2007 Aug).
There is also a nice review by Derewenda about protein engineering to enhance crystallizability and improve crystal properties.
Application of protein engineering to enhance crystallizability and improve crystal properties.
Acta Crystallogr D Biol Crystallogr. 2010 May;66(Pt 5):604-15.
Thanks for this feedback, Luc!