About the Author - Peter Nollert

Peter Nollert

I'm Peter Nollert and I write this blog to point researchers to topics that are relevant to protein crystallization. My mission is to help spread knowledge that is 'out there on the web' and help you succeed with your protein structure research.  I oversee the membrane protein research and technology development activities at Emerald BioStructures. Check out The GPCR blog, or my publications

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Protein Crystallization Hits

Protein Crystallization "Tips and Tricks" now in Acta Cryst F

by Peter Nollert
May 25, 2010 17:00

Kudos to Acta Cryst. F! Acta Cryst F has announced last year that "Tips and Tricks for Structural Biologists" can be published as stand-alone, regular papers under the category "Laboratory Communication".

Finally there is a forum that hosts peer-reviewed crystallization method reports (more in the Editorial ). I can't wait to see highly detailed descriptions of "special methods, equipment modifications, techniques for accomplishing certain tasks etc."

 

These 'How-to'-type reports should be very useful to all crystallizers practicing the science of protein crystallization. So far there have been several useful reports: one on heavy atom derivatization, two papers on protein crystal UV imaging and one on distinguishing salt vs. protein crystals with a melt test:

T. Beck, C. E. da Cunha and G. M. Sheldrick
How to get the magic triangle and the MAD triangle into your protein crystal
Acta Cryst. (2009). F65, 1068-1070

Synopsis: The handling of the phasing tools I3C and B3C is described, emphasizing practical aspects such as the preparation of solutions and incorporation of the compounds into protein crystals.

 

K. Dierks, A. Meyer, D. Oberthür, G. Rapp, H. Einspahr and C. Betzel
Efficient UV detection of protein crystals enabled by fluorescence excitation at wavelengths longer than 300 nm
Acta Cryst. (2010). F66, 478-484

Synopsis: Excitation of intrinsic fluorescence at wavelengths longer than 300 nm is effective in the detection of protein crystals in crystallization trials set up in the most commonly used hardware.

 

H.S. Gill
Evaluating the efficacy of tryptophan fluorescence and absorbance as a selection tool for identifying protein crystals

Acta Cryst. (2010). F66, 364-372
Synopsis: The effectiveness of using ultraviolet microscopes to illuminate protein crystals in high throughput screens is evaluated.

 

K. Raghunathan, P. T. Harris and D. N. Arvidson

Trial by fire: are the crystals macromolecules? 

Acta Cryst. (2010). F66, 615-620 
Synopsis: A simple `melt test' to distinguish salt crystals from macromolecule crystals is described.

 

Protein crystallization tips and tricks announcement taken from the IUCR mag.

Cheers,

Peter

Comments (3) -

11/21/2010 6:32:10 AM #

Great development! Smart move by the journal, now it becomes a "must read" for all practicing crystallographers

ho

11/21/2010 6:32:41 AM #

nice

ana

11/21/2010 6:33:42 AM #

My comment refers to "Efficient UV detection ....." by K. Dierks et al, Acta Cryst.(2010). F66, 478-484. I provide here some useful information for efficient detection of UV, visible, and near infrared radiations with very low quantum yield. The two optical techniques that I developed with narrow band optical filters and a pair of sheet polarizers greatly helped in the successful detection of UV for the first time from radioisotopes and XRF sources. I quote from the following research paper published in a peer reviewed journal. "Unexpected detection of UV dominant optical radiation owes to the use of bare PMT (9635QB, Thorn EMI) on which the source was directly kept, setting gain of the linear amplifier relatively higher than the requirement for a scintillation detector and time constant at 0.1 µs. Despite high gain setting, the optimally low background rate (12 cps) of the PMT noticed throughout the experiments ensured its satisfactory operating  
condition free from any light leak".  
  
M.A. Padmanabha Rao  
UV dominant optical emission newly detected from radioisotopes and XRF sources  
Brazilian Journal of Physics, Vol.40, no.1, March 2010.  
http://www.sbfisica.org.br/bjp/files/v40_38.pdf.

ana

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