by Peter Nollert
April 6, 2010 14:00
Since I've been asked, here's a list of protein crystallization practices that may be useful to reduce your work load for protein crystallization:
Spend less time with purification - set up trials with semi-pure samples that are difficult to purify any further:
Dirty protein - is purity overrated? Just be aware that this may backfire - since there are reports of accidential protein crystallizations: AcrB and OmpF may just be the tips of the iceberg.
Skip following up false-positive crystallizations :
The single most useful practice to reduce effort in protein crystallization
Eliminate the sample concentration step: concentrate in-situ:
Avoiding the protein concentration step prior to setting up crystallization experiments
Wait longer by giving your protein crystallization experiments more time:
Protein Crystallization by dehydration
Don't work on target proteins that don't crystallize and focus on those that are easy to crystallize:
The biggest SECRET ever for success in protein crystallization
Avoid loosing precious protein samples by storing them properly:
Best Practices for storing protein samples
Happy Spring Break,
Peter