Accelerate your research on protein function with parallel purification. The Protein Maker workstation makes high throughput protein purification chromatography simple by allowing you to run 24 columns (1-5 ml) in parallel, and process 96 samples unattended. Protein Maker’s flexible design allows you to customize sample and column types to achieve a variety of tasks:
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Evaluate gene expression levels
- Analyze functions of different isotypes or clones
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Determine the best protein or enzyme purification method for scaling up
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Screen constructs for protein structure studies
Instruments cannot be ordered online. Please contact a sales representative.
The Protein Maker's flexible design makes it easy to begin with wide-ranging proteomics projects to those that focus on a single gene product. The Protein Maker is used by Emerald BioStructures’ structural biology scientists for high throughput production of highly pure protein for X-ray crystallography.
Produce more protein, more flexibly, with the Protein Maker.
Images: Protein Maker was used to purify 6 surface mutant variants of recombinant proteins expressed in E. coli. His6-tagged proteins were purified through two rounds of Ni++ immobilized metal chelate chromatography (IMAC).
Left: Flow through fractions. Center: Eluted His6-tagged protein from Round 1. Right: From Round 2 of IMAC.
M= molecular weight standards
L= total loaded sample of construct 1 to illustrate total E. coli lysate
Parallel Protein Chromatography
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Purify up to 288 protein samples per day (more typical is four runs per day for about 100 samples per day). Parallel purification!
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Set up and walk away
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Configure your own sample preparation protocol
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Load large volumes
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Run 24 chromatography columns (1-5 ml) in parallel
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Harvest multiple mg amounts
Applications
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Antibody purification from 250 ml start volume (i.e. Protein A affinity chromatography)
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Biomarker discovery and sample preparation for mass spectrometry using immuno-depletion resins (blood plasma or cerebrospinal fluid)
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Parallel expression and parallel purification testing of (surface) mutagenized protein constructs (i.e. Ni-chelate chromatography)
Technical features
Accepts any standard 1 ml or 5 ml chromatography column
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High-throughput parallel protein purification for:
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Production: 24 different protein samples, one type of chromatographic resin
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Scouting: 24 different chromatographic resins, one protein sample
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Systematic two-step grid purification (2D chromatography)
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Compatible with a wide range of volumes (i.e. load 250 ml, elute 0.5 ml)
Links and Documentation
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Protein Maker Video. See the Protein Maker in action and see examples of applications.
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Protein Maker Brochure (PDF). Overview of the Protein Maker’s features and benefits.
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Protein Maker Details and Specifications (PDF) Protein Maker technical specifications and details.