Accelerate your research on protein function with parallel purification. The Protein Maker workstation makes high throughput protein purification chromatography simple by allowing you to run 24 columns (1-5 ml) in parallel, and process 96 samples unattended. Protein Maker’s flexible design allows you to customize sample and column types to achieve a variety of tasks:

• Evaluate gene expression levels

• Analyze functions of different isotypes or clones

• Determine the best protein or enzyme purification method for scaling up

• Screen constructs for protein structure studies

  Emerald Bio's new ProteinMaker 2.0 Software has a simulation mode that lets researchers invent, test, edit and archive new protein purification methods. In addition, the software upgrade includes Emerald Bio's innovative methods library. Besides enabling full control over the instrument's syringe pumps, valves and robotic XYZ gantry, the new software upgrade gives scientists the ability to pause any process whenever they want. The instrument easily adapts to a user's unique needs.

Instruments cannot be ordered online. Please contact a sales representative.

The Protein Maker's flexible design makes it easy to begin with wide-ranging proteomics projects to those that focus on a single gene product. The Protein Maker is used by Emerald BioStructures’ structural biology scientists for high throughput production of highly pure protein for X-ray crystallography.

Produce more protein, more flexibly, with the Protein Maker.

Images: Protein Maker was used to purify 6 surface mutant variants of recombinant proteins expressed in E. coli. His6-tagged proteins were purified through two rounds of Ni++ immobilized metal chelate chromatography (IMAC).

Left: Flow through fractions. Center: Eluted His6-tagged protein from Round 1. Right: From Round 2 of IMAC.

M= molecular weight standards

L= total loaded sample of construct 1 to illustrate total E. coli lysate

Parallel Protein Chromatography

• Purify up to 192 protein samples per day (more typical is four runs per day for about 100 samples per day). Parallel purification!

• Fully automated: Set up and walk away

• Customizable purification methods.

• Load range large volumes: A few mL or 1L+

• Run 24 chromatography columns in parallel

• 1-100+ mg scale purification

Applications

• Antibody purification from 250+ ml start volume 

• Risk-free purification scale-up through parallelization

• Multi-construct design for structural biology

• Lysis buffer scouting

Operational Modes

Links and Documentation

• Protein Maker Brochure (PDF). Overview of the Protein Maker’s features and benefits.

• Protein Maker Details and Specifications (PDF) Protein Maker technical specifications and details.

• Peer-reviewed Paper on Lysis Scouting in Acta Crys.