Primary Protein Crystallization Matrices
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| Highly effective random sparse matrices for the crystallization of biological macromolecules (proteins, nucleic acids, peptides and combinations thereof). Sixteen different crystallants and eleven different buffers, ranging from pH 4.5 to pH 10.5, ensure a broad sampling of crystallization space. |
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| For high-throughput crystallization try our Wizard Matrix Blocks! Order Wizard I and Wizard II in 96-well block plates. Each block includes a full Wizard I and Wizard II kit with 1.0 ml of each of the 96 formulations. Using a multi-channel pipette and one of our standard 96-well crystallization plates, you can rapidly set up crystallization experiments. |
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| Random sparse matrix for the crystallization of biological macromolecules (proteins, nucleic acids, peptides and combinations thereof). This solution set is comprised of 48 published formulations, which have been proven highly effective for use in the initial screening of biological macromolecules (Page, R. et al. Acta Cryst (2003) D59, 1028-1037; Wooh, J.W., Kidd, R.D., Martin, J.L., and Kobe, B. Acta Cryst (2003) D59, 769-772). These regents are designed as a starting point in the "shotgun" crystallization strategy, wherein a wide range of crystallants, buffers and salts are included in order to cover a broad range of crystallization space, but without the usual redundancies found in many commercial screening kits. In combination with the Wizard I and II crystal growth matrices (Wizard Suite), or as a stand-alone kit, Wizard III is designed to increase your probability of producing crystals during the "coarse screening" phase of the crystallization process. |
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| Precipitant Synergy uses synergistic combinations of mechanistically distinct precipitating agents to obtain crystallization hits from a primary set of 64 formulations. Each Precipitant Synergy (PS) formulation contains a carefully selected combination of at least two distinct precipitating agents including: (i) salts that affect the activity coefficient of water at high concentrations; (ii) organic solvents which alter the dielectric constant of the solvating medium; and (iii)PEGs which operate by increasing molecular crowding. Tests with 10 different proteins showed that the Precipitant Synergy screen roughly triples the number of different crystals obtained compared to other screens (Majeed, S., Ofek, Gl, Balachew, A., Huang, C.C., Zhou, Tl, Kwong, P.D. (2003) Structure, 11:1-20]. Patent Pending |
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| Sparse matrices for the crystallization of biological macromolecules. Every Cryo™ formulation will flash-freeze to a clear amorphous glass in liquid nitrogen or in the cryo-stream at 100k. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Crystals can be frozen directly from their growth chambers, thus avoiding the additional step of pre-equilibration with an artificial cryo-solvent that can damage the crystal. |
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| The Ozma PEG-Ion Series 48-Salt Screens are formulated using one of four different Mwt PEG's; 30% (w/v) PEG-1000, 20% (w/v) PEG-4000, 20% (w/v) PEG-8000, or 10% (w/v) PEG-10000 plus 48 different salts. The cationic components of the salts include coverage of Ammonium, Calcium, Lithium, Potassium, Sodium, Magnesium, and Zinc. The anionic components of the salts include coverage of Acetate, Chloride, Citrate, Fluoride, Formate, Iodide, Nitratte, Phosphate (mono-and di-basic), Sulfate, Tartrate and Thiocyanate. These screens can be used in combination with the buffers ranging in pH from 2.4 to 11.6 that comprise our pHat™ Buffer Screen to further expand the crystallization possibilities. |
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| A random sparse matrix for the crystallization of biological macromolecules that are embedded within a lipidic cubic phase (LCP) host matrix. Soluble and membrane proteins can be crystallized with the LCP formulation matrix. Every formulation is compatible with a monolein-based lipidic cubic phase under conditions typically used for this type of crystallization experiment (i.e. 200 nanoliters of 60% monolein-based lipidic cubic phase and 1 microliter of formulation). |
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| The complete LCP kit contains all tools, formulations, and lipid to prepare micro-crystallization experiments according to the LCP micro method. Biological macromolecules are first incorporated into a lipidic material (LCP), and in a second step the LCP is dispensed in portions of approximately 200 nanoliters. The LCP forms spontaneously and acts as a matrix supporting the crystallization of soluble and membrane proteins. A detailed protocol explains the preparation of the LCP, its dispensation, and the set-up of crystallization experiments. |
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Optimization Screens
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| The pHat™ Buffer screen is a set of 96 solutions containing 12 different buffer systems at 8 different pHs, ranging from +/- 1.4pH units around the pKa. The screen covers the entire crystallization space with buffers ranging from pH 2.4 to pH 11.6. The set of buffers is designed to complement the use of any of our 96 formulation crystal growth matrices and can be employed to add another dimension to the search for optimal protein crystal growth conditions. The buffers can be applied to your protein crystallization hits to simultaneously explore the effects of pH and buffer composition on crystal growth. All Emerald BioSystems' matrices include instruction and technical sheets. |
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The ADDit™ Additives Screen contains 96 different small molecule and salt solutions that have been found to be effective in aiding the crystallization of biological macromolecules. It comprises a range of crystal growth promoters and nucleation suppressants including detergents, chelators, reducing agents, volatile and non-volatile organics, heavy atoms and salts. The purpose of these solutions is to increase the quality and/or size of initial crystal hits when added to a lead condition. The additives are delivered in a 96 well block containing 1 ml of solution in each well at 10x the recommended final concentration. The block is designed for easy use with a single or multi-channel pipette, the latter allowing for rapid setup of a full 96 formulation optimization screen. Furthermore, the additives are organized in the block according to category in order to facilitate the setup of subsequent refinement screens that lack some of the additive classes.
The preferred use of this screen follows this scheme:
- Identify a non-perfect hit (i.e. microcrystals, weakly diffracting crystals, shiny precipitate.)
- Supplement 96 identical crystallization experiments with 10% (v/v) of additive screen component.
- Inspect and note change in morphology of crystals.
- Use the improved crystals for diffraction or follow up hits with changed morphology with a one-dimensional fine scree (e.g vary additive component from 0.5, 2, 5, 8, 15 to 20% (v/v)).
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eScreens
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Custom Screens that you design.
Custom crystallization screens are just a click away. Your custom screen will be made by the Emerald BioSystem's Matrix Maker™ robot.
You choose the number of conditions.
You select from our list of reagents (i.e. PEGs, salts, organic solvents, detergents, buffers etc.). If you need an ingredient not on our list, just let us know.
Tubes or blocks, all prepared especially for you!
Use our Excel spreadsheet or send us your own via email. We will verify your order and a representative will contact you with a price.
 E-screen data
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Crystallization Consumables
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