Highly effective random sparse matrices for the crystallization of biological macromolecules (proteins, nucleic acids, peptides and combinations thereof). Sixteen different crystallants and eleven different buffers, ranging from pH 4.5 to pH 10.5, ensure a broad sampling of crystallization space. Back to Top Tech Sheet Downloads
	For high-throughput crystallization try our Wizard Matrix Blocks! Order Wizard I and Wizard II in 96-well block plates. Each block includes a full Wizard I and Wizard II kit with 1.7ml of each of the 96 formulations. Using a multi-channel pipette and one of our standard 96-well crystallization plates, you can rapidly set up crystallization experiments.  Back to Top Tech Sheet Downloads
	Random sparse matrix for the crystallization of biological macromolecules (proteins, nucleic acids, peptides and combinations thereof). This solution set is comprised of 48 published formulations, which have been proven highly effective for use in the initial screening of biological macromolecules (Page, R. et al. Acta Cryst (2003) D59, 1028-1037; Wooh, J.W., Kidd, R.D., Martin, J.L., and Kobe, B. Acta Cryst (2003) D59, 769-772). These regents are designed as a starting point in the "shotgun" crystallization strategy, wherein a wide range of crystallants, buffers and salts are included in order to cover a broad range of crystallization space, but without the usual redundancies found in many commercial screening kits. In combination with the Wizard I and II crystal growth matrices, or as a stand-alone kit, Wizard III is designed to increase your probability of producing crystals during the "coarse screening" phase of the crystallization process. Back to Top Tech Sheet Downloads
	Precipitant Synergy uses synergistic combinations of mechanistically distinct precipitating agents to obtain crystallization hits from a primary set of 64 formulations. Each Precipitant Synergy (PS) formulation contains a carefully selected combination of at least two mechanistically distinct precipitating agents including: (i) salts that affect the activity coefficient of water at high concentrations; (ii) organic solvents which alter the dielectric constant of the solvating medium; and (iii)PEGs which operate by increasing molecular crowding. Tests with 10 different proteins showed that the Precipitant Synergy screen roughly triples the number of different crystals obtained compared to other screens (Majeed, S., Ofek, Gl, Balachew, A., Huang, C.C., Zhou, Tl, Kwong, P.D. (2003) Structure, 11:1-20]. Patent Pending Back to Top Tech Sheet Downloads EXP192 Primary Block Exp64 33% Block Exp64 33% Tubes Exp64 67% Block Exp64 67% Tubes Primary64 Block Primary64 Tubes
	Sparse matrices for the crystallization of biological macromolecules. Every Cryo™ formulation will flash-freeze to a clear amorphous glass in liquid nitrogen or in the cryo-stream at 100k. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Crystals can be frozen directly from their growth chambers, thus avoiding the additional step of pre-equilibration with an artificial cryo-solvent that can damage the crystal.  Back to Top Tech Sheet Downloads
	Setting up experiments has just gotten easier with our Cryo matrix blocks. Order Cryo I and Cryo II in 96-well block plates. Each block includes a full Cryo kit with 1.7ml of each of the 96 formulations. Using a multi-channel pipette and one of our standard 96-well block plates, you can rapidly set up crystallization experiments.  Back to Top Tech Sheet Downloads
	The Ozma PEG-Ion Series 48-Salt Screens are formulated using one of four different Mwt PEG's; 30% (w/v) PEG-1000, 20% (w/v) PEG-4000, 20% (w/v) PEG-8000, or 10% (w/v) PEG-10000 plus 48 different salts. The cationic components of the salts include coverage of Ammonium, Calcium, Lithium, Potassium, Sodium, Magnesium, and Zinc. The anionic components of the salts include coverage of Acetate, Chloride, Citrate, Fluoride, Formate, Iodide, Nitratte, Phosphate (mono-and di-basic), Sulfate, Tartrate and Thiocyanate. These screens can be used in combination with the buffers ranging in pH from 2.4 to 11.6 that comprise our pHat™ Buffer Screen to further expand the crystallization possibilities.  Back to Top Tech Sheet Downloads
	The PEG Anomalous crystallization screens combine the design of the Ozma PEG-Ion with 48 different anomalous salts that have a heavy atom ion component (cation or anion) with strong anomalous X-ray scattering behavior within the wavelength range generally useful for macromolecular crystallography (0.9 - 2.3 Angstrom). These heavy ions have been shown to bind non-covalently with high occupancy to the surfaces of protein molecules in crystals, providing an excellent means for single-crystal phasing via their anomalous scattering signal. In the last decade, the use of Multi-wavelength Anomalous Dispersion (MAD) has become a routine method of phase determination in macromolecular crystallography. A necessity of the MAD method has been the requirement of tunable synchrotron radiation sources for data collection at multiple wavelengths. Recent advances in data collection techniques and phasing algorithms now allow accurate phase determination from Single-wavelength Anomalous Diffractions (SAD) experiments. In additional, incorporation of non-covalently bound ions into crystals of macromolecules is applicable to a wide variety of anomalous scattering elements, many of which have strong anomalous signals at the wavelength of copper Κα radiation (λ =1.5418 Angstrom) produced by laboratory X-ray generators. The availability of chromium Κα (λ=2.2909) radiation from recently introduced chromium rotating anodes allows even stronger anomalous scattering signals to be observed from some elements with laboratory X-ray generators. The PEG Anomalous Screens contain 48 formulations and are delivered in a 96-well block plate containing 1 ml of each formulation. Select  Select  Peg 1k Peg 1k Peg 4k Peg 4k Peg 8k Peg 8k Peg 10k Peg 10k Full Series Full Series
	A random sparse matrix for the crystallization of biological macromolecules that are embedded within a lipidic cubic phase (LCP) host matrix. Soluble and membrane proteins can be crystallized with the LCP formulation matrix. Every formulation is compatible with a monolein-based lipidic cubic phase under conditions typically used for this type of crystallization experiment (i.e. 200 nanoliters of 60% monolein-based lipidic cubic phase and 1 microliter of formulation).  Back to Top Tech Sheet Downloads
	The complete LCP kit contains all tools, formulations, and lipid to prepare micro-crystallization experiments according to the LCP micro method. Biological macromolecules are first incorporated into a lipidic material (LCP), and in a second step the LCP is dispensed in portions of approximately 200 nanoliters. The LCP forms spontaneously and acts as a matrix supporting the crystallization of soluble and membrane proteins. A detailed protocol explains the preparation of the LCP, its dispensation, and the set-up of crystallization experiments.  Back to Top Tech Sheet Downloads

Any Screen you want: Customized Optimization Screens

If you want an optimization screen from an Emerald BioSystems screen, just tell us the formulation for the condition or conditions that you want to optimize. For any lead conditions, whether from an Emerald BioSystems screen or not, we can make your desired optimization screen through several different methods using our Crystal Miner software. Standard grid screening optimization screens are made using our software's Component Variation feature. Gradients can be designed based on pH and concentrations for all components in the lead condition. Reagents not present in the lead condition can also be added to the optimization screen. The image to the left is an example of the Component Variation Optimization of Wizard condition #28 to make a new 24-condition screen.

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