Wizard: Precipitant Synergy Crystallization Screen

Application: Wizard Precipitant Synergy formulations in blocks are particularly well suited for high-throughput protein crystallization in 96 well standard formats, using multichannel pipettors or robotic dispensation instrumentation.

Recommended Use Depending on the type of crystallization tray and employed reservoir volume, a single Wizard Precipitant Synergy protein crystallization kit provides formulations for ca. 12 to 24 96-well based crystallization plates (assuming reservoir well volumes of 160 or 80 uL). 

Approximate total number of crystallization setups: 1,152 – 2,304 corresponding to six or twelve unique 192 formulation crystallization trials. 

Dimensions: Well positions of deep well block plates conform to well center positional requirements for microplates (ANSI/SBS 4-2004).

Well shape: round

Well diameter: 7mm
Well depth: 35mm
Well bottom shape: round
Block dimensions: 128 x 86 x 42 mm
Formulations arrangement:  64 base Precipitant Synergy formulations are arranged sequentially in each row, with every third well filled with a unique set of reagents. Three neighboring wells in rows each contain a base formulation consisting of 100%, 67% and 33% of precipitation reagents and constant buffer concentrations of 100 mM.

Wizard: Precipitant Synergy Block Tech Sheet

Application: Precipitant Synergy formulations are used to prepare protein crystallization experiments in a variety of experiment formats: including vapor diffusion or batch crystallization, sitting or hanging drop configurations.

Recommended Use: Primary protein crystallization trials, particularly difficult-to-crystallize proteins.

Primary screening of one target protein using a total of 192 different crystallization formulations. Simple coverage of 768 distinct crystallization conditions can be achieved by setting up crystallization experiments employing two protein concentrations (i.e. 20 and 10 mg/mL) and incubation at 4ºC and room temperature.

Long Term Storage: Room temperature

Use / Stability: Kit formulations are stable for one year from date of receipt

Handling: Avoid freezing. Avoid Temperatures above 37ºC. Protect from light. Once opened, reseal with tape to reduce evaporation.

Validation: Crystallization tests showed that the use of Wizard Precipitant Synergy formulations “…significantly enhanced both the probability of crystallization as well as the quality of optimized crystals”. Tests with several difficult to crystallize proteins showed a significant two to three-fold increase in the overall probability of crystallization (Mayeed et al., 2003).

Literature References Majeed, S., Ofek, G., Belachew, A., Huang, C.C., Zhou, T., Kwong, P.D. (2003) Enhancing protein crystallization through precipitant synergy. Structure, 11: 1-20

US patent: 7,300,520

Wizard: Precipitant Synergy Tech Sheet