Wizard: Cubic LCP Kit
E-mail this product to a friendThe tools in the Wizard: Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard: Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.
Wizard: Cubic LCP Block Tech sheet
The Wizard: Cubic LCP Kit™ kit contains all tools, formulations, and lipid to prepare micro-crystallization experiments according to the LCP micro method. To use this kit, the biological macromolecule sample, typically a membrane protein, is first incorporated into a lipidic material (LCP) and then this resulting LCP material can be dispensed in portions of approximately 200 nanoliters. The LCP forms spontaneously and acts as a matrix supporting the crystallization of soluble and membrane proteins.
The Wizard: Cubic™ Screen random sparse matrix is designed for the crystallization of biological macromolecules that are embedded within a lipidic cubic phase (LCP) host matrix. Every formulation in this 96-well plate is compatible with monoolein-based lipidic cubic phases.
96 formulations, 1.7 mL volumes in a 96 well block plate.
Wizard: Cubic Screen Technical Sheet
Matrix lipid for crystallization within Lipidic Cubic Phases. This lipid forms a Lipidic Cubic Phase (LCP) material spontaneously when mixed with water (or your protein solution) at a 60 (monoolein) : 40 (aqueous) volume ratio.
10 uL Microsyringe pipette for use in lipidic cubic phase (LCP) work
This asymmetric mixer union couples to 250 uL syringes on one side and to 250 uL, 50 uL or 10 uL on the opposing side. This allows for simple lipidic cubic phase (LCP) generation using two 250 uL syringes and transfer into a 10 uL syringe for small volume LCP dispensation.
This symmetric mixer union couples to 50 uL and 10 uL syringes on both ends. Suitable for lipidic cubic phase (LCP) generation, mixing and transfer of LCP into 10 uL syringes.
Syringe for the preparation of lipidic cubic phase (LCP).
Syringe for the preparation of lipidic cubic phase (LCP).
These polystyrene trays have 72 batch-type wells for batch-crystallization ‘under oil’ or for crystallization within lipidic cubic phases (LCP). Offers excellent visibility to your experiment, seals well with tape, and offers simple access to the crystallization setup. 72 wells arranged in 6 columns and 12 rows.
Pack of 10.