(ENDEXT) WEPRO1240G Expression Kit includes:
WEPRO 1240G*-240 OD (1.0 ml x 2)
pEU-E01-MCS Vector
pEU-E01-DHFR (for positive control)
SUB-AMIX (40x, 1mL x 4)
SP6 RNA Polymerase (80,000 u/mL, 30uL)
RNase Inhibitor (80,000 u/mL, 30uL)
NTPs Mix (25mM, 200uL)
5x Transcription Buffer (400uL)
Creatine Kinase (20uL) recommended to be used once
This Kit contains enough material for one run of the Protemist DT II robot with 6 samples. Alternatively, this kit can be used manually, to carry out 96 expression reactions in a 96-well plate.
Recommended Storage of the WEPRO 1240G at -80 °C and the other reagents at -20 °C.
* WEPRO 1240G is optimized to produce high-purity samples of GST-tagged proteins.
CFS-TRI-1240G