(ENDEXT) WEPRO1240H Expression Kit includes:
WEPRO 1240H*-240 OD (1.0 ml x 2)
pEU-E01-MCS Vector
pEU-E01-DHFR (for positive control)
SUB-AMIX (40x, 1mL x 4)
SP6 RNA Polymerase (80,000 u/mL, 30uL)
RNase Inhibitor (80,000 u/mL, 30uL)
NTP Mix (25mM, 200uL)
5x Transcription Buffer (400uL)
Creatine Kinase (20uL) recommended to be used once
This Kit contains enough material for one run of the Protemist DT II robot with 6 samples. Alternatively, this kit can be used manually, to carry out 96 expression reactions in a 96-well plate.
Recommended Storage of WEPROH 1240 -80 °C and the other reagents -20 °C.
* WEPRO 1240H is optimized to produce high-purity samples of His-tagged proteins.
CFS-TRI-1240H